{rfName}
Al

Indexed in

License and use

Citations

64

Altmetrics

Analysis of institutional authors

Fernández Larrea, Juan BautistaAuthor

Share

Publications
>
Article

Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus

Publicated to:Cancer Research. 63 (17): 5178-5187 - 2003-09-01 63(17), DOI:

Authors: Guil, S; de La Glesia, N; Fernández-Larrea, J; Cifuentes, D; Ferrer, JC; Guinovart, JJ; Bach-Elias, M

Affiliations

- Author
Consejo Superior de Investigaciones Científicas - Author

Abstract

We characterized a novel protein of the Ras family, p19 (H-RasIDX). The c-H-ras proto-oncogene undergoes alternative splicing of the exon termed IDX. We show that the alternative p19 mRNA is stable and as abundant as p21 (p21 H-Ras4A) mRNA in all of the human tissues and cell lines tested. IDX is spliced into stable mRNA in different mammalian species, which present a high degree of nucleotide conservation. Both the endogenous and the transiently expressed p19 protein are detected in COS-1 and HeLa cells and show nuclear diffuse and speckled patterns as well as cytoplasmic localization. In yeast two-hybrid assays, p19 did not interact with two known p21 effectors, Raf1 and Rin1, but was shown to interact with RACK1, a scaffolding protein that promotes multiprotein complexes in different signaling pathways. This observation suggests that p19 and p21 play differential and complementary roles in the cell.

Keywords

AnimalsBase sequenceCell nucleusCytoplasmHumansMetabolismMiceMolecular sequence dataRabbitsRatsSpain

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Cancer Research due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2003, it was in position 7/119, thus managing to position itself as a Q1 (Primer Cuartil), in the category Oncology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-06-26:

  • WoS: 33
  • Europe PMC: 31

Impact and social visibility

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United States.