{rfName}

Indexed in

License and use

Icono OpenAccess

Citations

2

Altmetrics

Analysis of institutional authors

Marqués MAuthorGarcía-Sayago FAuthor

Share

February 27, 2023
Publications
>
Article
Sí

Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants

Publicated to:Methodsx. 10 (102069): 102069- - 2023-02-16 10(102069), DOI: 10.1016/j.mex.2023.102069

Authors: Gutierrez-Martin, Daniel; Marques, Montse; Pons-Escoda, Albert; Vidal, Noemi; Bruna, Jordi; Restrepo-Montes, Esteban; Lopez-Serna, Rebeca; Garcia-Sayago, Francisco; Majos, Carles; Gago-Ferrero, Pablo; Gil-Solsona, Ruben

Affiliations

Autonomous Univ Barcelona, Inst Neurosci, Biomed Res Networking Ctr Neurodegenerat Dis CIBER, Dept Cell Biol Physiol & Immunol, Bellaterra, Spain - Author
CSIC - Instituto de Diagnostico Ambiental y Estudios del Agua (IDAEA) - Author
Hosp Bellvitge Princeps Espanya, Dept Pathol, Barcelona 08907, Spain - Author
Hosp Univ Bellvitge ICO LHospitalet, Neurooncol Unit, Barcelona 08907, Spain - Author
Hospital Universitari de Bellvitge - Author
Inst Diagnost Imatge IDI, Dept Radiol, Barcelona 08907, Spain - Author
Inst Invest Biomed Bellvitge IDIBELL, Barcelona 08907, Spain - Author
Inst Invest Sanitaria Pere Virgili IISPV, Reus, Spain - Author
Inst Med Legal Catalunya & Ciencies Forenses, Dept Justicia, Tarragona, Spain - Author
Inst Sustainable Proc, Dr Mergelina S-N, Valladolid 47011, Spain - Author
Institut d'Investigació Sanitària Pere Virgili , Universitat Rovira i Virgili - Author
Institute of Sustainable Processes , Universidad de Valladolid - Author
Instituto de Neurociencias, Universitat Autònoma de Barcelona , Hospital Universitari de Bellvitge - Author
Spanish Council Sci Res CSIC, Inst Environm Assessment & Water Res IDAEA, Severo Ochoa Excellence Ctr, Jordi Girona 18-26, E-08034 Barcelona, Spain - Author
Univ Rovira & Virgili, Sch Med, Lab Toxicol & Environm Hlth, St Llorenc 21, Reus 43201, Catalonia, Spain - Author
Univ Valladolid, Fac Sci, Dept Analyt Chem, Valladolid 47011, Spain - Author
Universitat Rovira i Virgili , Institut de Medicina Legal i Ciències Forenses - Author
See more

Abstract

Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).

Keywords

bead beatinghuman biomonitoringlc-hrmsnon-target screeningsuspect screeningBead beatingExposomeHuman biomonitoringHuman tissueLc-hrmsNon-target screeningSuspect screening

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Methodsx due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 59/134, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Multidisciplinary Sciences. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Medical Laboratory Technology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-16:

  • Scopus: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-16:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 9.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 7.4.
  • The number of mentions on the social network X (formerly Twitter): 9 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.11797/imarina9292125