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Analysis of institutional authors

Sánchez-Martin SAuthorAltuna-Coy AAuthorArreaza-Gil VAuthorBernal-Escoté XAuthorAscaso-Til HAuthorSegarra-Tomás JAuthorRuiz-Plazas XAuthor
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Tumoral periprostatic adipose tissue exovesicles-derived miR-20a-5p regulates prostate cancer cell proliferation and inflammation through the RORA gene.

Publicated to:Journal Of Translational Medicine. 22 (1): 661- - 2024-07-15 22(1), DOI: 10.1186/s12967-024-05458-3

Authors: Sánchez-Martin S; Altuna-Coy A; Arreaza-Gil V; Bernal-Escoté X; Fontgivell JFG; Ascaso-Til H; Segarra-Tomás J; Ruiz-Plazas X; Chacón MR

Affiliations

Disease Biomarkers and Molecular Mechanisms Group. IISPV. Joan, XXIII University Hospital, Universitat Rovira i Virgili, Tarragona, Spain. - Author
Disease Biomarkers and Molecular Mechanisms Group. IISPV. Joan, XXIII University Hospital, Universitat Rovira i Virgili, Tarragona, Spain. mrch2424@gmail.com. - Author
Urology Unit, Joan XXIII University Hospital, Tarragona, Spain. - Author

Abstract

From the first steps of prostate cancer (PCa) initiation, tumours are in contact with the most-proximal adipose tissue called periprostatic adipose tissue (PPAT). Extracellular vesicles are important carriers of non-coding RNA such as miRNAs that are crucial for cellular communication. The secretion of extracellular vesicles by PPAT may play a key role in the interactions between adipocytes and tumour. Analysing the PPAT exovesicles (EVs) derived-miRNA content can be of great relevance for understanding tumour progression and aggressiveness. A total of 24 samples of human PPAT and 17 samples of perivesical adipose tissue (PVAT) were used. EVs were characterized by western blot and transmission electron microscopy (TEM), and uptake by PCa cells was verified by confocal microscopy. PPAT and PVAT explants were cultured overnight, EVs were isolated, and miRNA content expression profile was analysed. Pathway and functional enrichment analyses were performed seeking potential miRNA targets. In vitro functional studies were evaluated using PCa cells lines, miRNA inhibitors and target gene silencers. Western blot and TEM revealed the characteristics of EVs derived from PPAT (PPAT-EVs) samples. The EVs were up taken and found in the cytoplasm of PCa cells. Nine miRNAs were differentially expressed between PPAT and PVAT samples. The RORA gene (RAR Related Orphan Receptor A) was identified as a common target of 9 miRNA-regulated pathways. In vitro functional analysis revealed that the RORA gene was regulated by PPAT-EVs-derived miRNAs and was found to be implicated in cell proliferation and inflammation. Tumour periprostatic adipose tissue is linked to PCa tumour aggressiveness and could be envisaged for new therapeutic strategies.

Keywords
Adipose tissueCell line, tumorCell proliferationExovesiclesExtracellular vesiclesGene expression regulation, neoplasticHumansInflammationMaleMicrornasMirn20a microrna, humanMirnasPeriprostatic adipose tissueProstateProstate cancerProstatic neoplasmsRora gene

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Translational Medicine due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 29/189, thus managing to position itself as a Q1 (Primer Cuartil), in the category Medicine, Research & Experimental.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-05-22:

  • Scopus: 1
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-22:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 10.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.7.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Sanchez Martin, Silvia) and Last Author (Chacón MR).