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This research was funded by the projects JPIW2013-095-C03-03 of MINECO (Spain), AQUAVALENS of the Seventh Framework Program (FP7/2007-2013) from the European Union, as well as the CONACYT project CB-2008-01-103142 (UAEM1900/2010C) and Secretaria de Investigacion y Postgrado (SIP-IPN) project 20211351, 20220818. OACM received grant-aided support from "Consejo Nacional de Ciencia y Tecnologia" (CONACyT, Mexico). GCE received support from the "Sistema Nacional de Investigadores" (SNI) from CONACyT, Mexico, Estimulo al Desempeno en Investigacion and Comision y Fomento de Actividades Academicas (Instituto Politecnico Nacional).

Impact on the Sustainable Development Goals (SDGs)

Analysis of institutional authors

Fernandez-Bravo, AnaAuthorFernández Bravo, AnaAuthorPérez-Cataluña, AlbaAuthorBeaz-Hidalgo, RoxanaAuthorJose Figueras, MariaCorresponding Author

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November 28, 2022
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First Record of the Rare Species Aeromonas lusitana from Rainbow Trout (Oncorhynchus mykiss, Walbaum): Comparative Analysis with the Existing Strains

Publicated to:Pathogens. 11 (11): 1299-1299 - 2022-11-01 11(11), DOI: 10.3390/pathogens11111299

Authors: Fernandez-Bravo, Ana; Vega-Sanchez, Vicente; Perez-Cataluna, Alba; Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Martinez-Murcia, Antonio; Soriano-Vargas, Edgardo; Alejandro Cabrero-Martinez, Omar; Castro-Escarpulli, Graciela; Jose Figueras, Maria;

Affiliations

Inst Politecn Nacl IPN, Lab Invest Clin & Ambiental, Escuela Nacl Ciencias Biol ENCB, Mexico City 11350, DF, Mexico - Author
Univ Autonoma Estado Hidalgo, Inst Ciencias Agr, Tulancingo 43600, Mexico - Author
Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Toluca 50295, Mexico - Author
Univ Miguel Hernandez, EPSO, Area Microbiol, Orihuela 03300, Spain - Author
Univ Rovira & Virgili, Fac Med & Ciencies Salut, Dept Ciences Med Basiques, Unitat Microbiol,IISPV, E-43201 Reus, Spain - Author
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Abstract

The species Aeromonas lusitana was first described in 2016 with five strains recovered from untreated water and vegetables from Portugal. Since then, no further records exist of this species. During a surveillance study on the presence of Aeromonas in fish farms in Mexico, a new strain (ESV351) of the mentioned species isolated from a rainbow trout was recovered. It was identified because it clustered phylogenetically with the type strain of A. lusitana based on the analysis of the ipoD gene sequences. In the present study, phenotypic characteristics, antimicrobial resistance profiles, and the presence of putative virulence genes of this novel strain (ESV-351) were determined in parallel to the five isolates from the original species description. Phenotypic differential characteristics exhibited by A. lusitana ESV-351 depicted an evident similarity to the characteristics exhibited by the other evaluated strains. However, the novel strain was positive for the production of indole using conventional methods, while the rest of the strains, including the type strain, were negative for its production. Furthermore, intermediate resistance to ampicillin, amoxicillin-clavulanic acid and cephalothin was detected in both the novel and the type strain. Five different virulence-related genes were detected in the novel strain and in the previously described strains, with the type strain exhibiting the highest number of virulence-related genes. In addition to this, the genome of the novel strain (ESV-351) was sequenced and compared with the genomes from the type strain (A. lusitana CECT 7828(T)) and other Aeromonas spp. The genomic analysis defined Aeromonas tecta as the closest species to A. lusitana with a highly similar number of predicted proteins. The genomic size, the number of protein-encoding genes and the number of different tRNAs, among other characteristics, make it possible to propose that the ESV-351 strain could potentially have the capacity to adapt to different environments. Genome comparison of the ESV-351 strain with the type strain revealed that both possess a similar sequence of the citrate synthase gene. In addition to this finding, the chromosomal region containing the citrate synthase locus of the novel strain exhibits some similarity to the chromosomal region in the genome of the A. hydrophila type strain and other known human pathogens, such as Vibrio cholerae. This could suggest a possible virulence role for the citrate synthase gene in A. lusitana (ESV-351).

Keywords

Aeromonas lusitanaAniCitrate synthaseCore genomesDiversityEscherichia-coliGenome sequenceGenus aeromonasIdentificationIsddhLife below waterSalmonicidaSp nov.Spp.TaxonomyVirulence genes

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Pathogens due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 62/135, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Microbiology. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Immunology and Microbiology (Miscellaneous).

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-10-21:

  • Open Alex: 1
  • Google Scholar: 1
  • Scopus: 1
  • Europe PMC: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-10-21:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 10.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 14 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.5.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: http://hdl.handle.net/20.500.11797/imarina9286946
Continuing with the social impact of the work, it is important to emphasize that, due to its content, it can be assigned to the area of interest of ODS 14 - Conserve and sustainably use the oceans, seas and marine resources for sustainable development, with a probability of 71% according to the mBERT algorithm developed by Aurora University.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Mexico.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Fernández Bravo, Ana) and Last Author (Figueras Salvat, María Josefa).

the author responsible for correspondence tasks has been Figueras Salvat, María Josefa.